Uso de espátula e dissector para otimização do Transplante Lamelar de Córnea (DALK ).
 
Gustavo Bonfadini^1,2,3; Eun Chul Kim^1,4; Mauro Campos³ Albert S. Jun ¹
 
1- Cornea & Anterior Segment Service, Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
2- Rio de Janeiro Eye Bank – INTO, Rio de Janeiro, RJ, Brazil.
3 Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
4- College of Medicine, Catholic University of Korea, Seoul, Korea.
 
Financial Support: none
This manuscript was not presented at any meeting.
The authors have no public and private financial support, or financial interest.
Running head: Novel spatula and dissector for DALK
 
Address reprint requests to: Albert S. Jun – Cornea and External Disease Service Wilmer Smith Building 5011, The Johns Hopkins Medical Institutions 400 N. Wolfe Street, Baltimore, MD 21231
 
ABSTRACT
Aims: We describe a novel spatula and dissector to facilitate the big-bubble technique in deep anterior lamellar keratoplasty (DALK). Methods: A 29-year-old man who was diagnosed with bilateral keratoconus underwent deep anterior lamellar keratoplasty (DALK). After 350µm partial thickness incision of the recipient cornea, the Bonfadini dissector was inserted at the deepest point in the peripheral incision and could be advanced to the center of the cornea safely because of its “semi-sharp” tip. After achieving the big-bubble (BB) separation of Descemet membrane (DM) from the overlying stroma, the anterior stromal disc was removed. Viscoelastic material was placed on the stromal bed to prevent uncontrolled collapse and perforation of DM during the paracentesis blade incision into the BB. We could detect the safe opening of the BB using the Bonfadini dissector by the leakage of air bubbles into the viscoelastic material. After injecting viscoelastic material into the BB space, we inserted the Bonfadini spatula into the big-bubble safely because of its curved profile and blunt edges. The groove along the length of the Bonfadini spatula enables safe and efficient incision or the residual stromal tissue using the pointed end of a sharp blade while protecting the underlying DM. After removal of posterior stroma, the donor button was sutured with 16 interrupted 10-0 nylon sutures. Results: This technique and the use of the Bonfadini spatula and dissector facilitate exposure of Descemet membrane. Conclusions: The smooth Bonfadini DALK spatula and dissectorfacilitate safe and efficient completion of DALK surgery.
 
Keywords:Deep anterior lamellar keratoplasty; Corneal transplantation/methods; Keratoplasty; Bonfadini dissector; Bonfadini spatula
 
 
RESUMO
Objetivo: Descrevemos o uso de novos instrumentais cirúrgicos para facilitar a técnica de “big-bubble” na ceratoplastia lamelar anterior profunda ( Transplante Lamelar de Córnea – DALK).Métodos: Paciente masculino, 29 anos, foi diagnosticado com ceratocone bilateral e submetido a ceratoplastia lamelar anterior profunda (DALK). Após incisão da córnea receptora numa profundidade de 350µm de espessura parcial, o dissector Bonfadini foi inserido no ponto mais profundo da incisão periférica e pode avançar para o centro da córnea com segurança devido à sua ponta “semi-afiada”. Depois de realizar a “big-bubble” (BB) e atingir a separação da Membrana de Descemet (MD) do estroma sobrejacente, o disco corneano de estroma anterior foi removido. Viscoelástico foi colocado sobre o leito do estroma remanescente para impedir o colapso não controlado e perfuração da MD durante a incisão na BB com lâmina de paracentese. Verificamos segurança no rompimento do estroma remanescente com o auxilio do dissector Bonfadini, para liberação da bolha de ar da BB através do viscoelástico. Depois de injetar o viscoelástico no espaço da BB, inserimos a espátula Bonfadini neste espaço, o que demosntrou-se seguro devido ao formato curvo e das bordas arredondadas do instrumental. A chanfradura ao longo do comprimento da espátula Bonfadini permite a incisão pela ponta de uma lâmina afiada, protegendo assim a MD subjacente. Após a remoção do estroma posterior, o botão doador foi suturado com 16 pontos interrompidos de fio nylon 10.0. Resultados: Esta técnica e o uso da espátula Bonfadini e dissector facilitam a exposição de membrana de Descemet. Conclusão: A superfície lisa da espátula Bonfadini e dissector, facilitam a realização segura e eficiente da ceratoplastia lamelar anterior profunda (DALK).
 
Descritores: Ceratoplastia lamelar anterior profunda; Transplante de córnea/métodos; Ceratoplastia; Dissector Bonfadini; Espatula Bonfadini
 
INTRODUCTION
Deep anterior lamellar keratoplasty (DALK) has been proposed as an alternative to penetrating keratoplasty (PK) for the treatment of various corneal diseases not affecting the endothelium. DALK surgery removes the anterior layers of the cornea, cleaving the deep stroma from Descemet membrane (DM). The advantages of the DALK technique for corneal stromal diseases include absence of endothelial rejection, avoidance of potential open-sky intra-operative complications of PK, faster visual rehabilitation due to earlier suture removal, and a predicted longer graft survival because of the lower rate of endothelial cell loss.¹
DALK is a time-consuming and technically demanding procedure. The most frequent intraoperative complication is perforation of DM while attempting to separate it from the overlying stroma during creation of the big-bubble (BB).²Sarnicola et al.³ reported that a smooth spatula and cannula can facilitate a high percentage of successful DALK and make the procedure more reliable than compared to air injection with a needle.
In this report, we describe a novel Bonfadini dissector and spatula for safely manipulating the big-bubble and removing posterior stroma.
 
Surgical technique
A 29-year-old man diagnosed with bilateral keratoconus 6 years ago, visited our service complaining of ocular pain and decreased vision in the right eye. He had worn rigid gas permeable (RGP) contact lenses for 16 years. His best spectacle corrected visual acuity OD was 20/80 and OS was 20/25. Slit-lamp examination revealed marked corneal stromal scarring and epithelial punctuate erosions on the right central cornea. Hence, deep anterior lamellar keratoplasty (DALK) was completed uneventfully in the right eye.
The operation was performed under sub-Tenon anesthesia by A.S.J; The technique described by Anwar et al.⁴was followed with the described modifications. A surgical marking pen was used to mark the center of the host cornea. An 8.5mm diameter trephine was used to lightly score the epithelium of the host cornea to outline the recipient bed. An astigmatic keratotomy blade was used to incise the recipient cornea to a depth of 350µm along the 8.5mm trephine mark. Through a small peripheral clear cornea paracentesis, the anterior chamber (AC) was filled with air, and approximately physiologic intraocular pressure confirmed.
The Bonfadini dissector (Katena Products, USA; Figure 1A) was inserted at the deepest point in the peripheral groove and was advanced toward the center of the cornea. Once the Bonfadini dissector was approximately 1-2mm from the apex of the cone (Figure 1B), it was removed and the Fogla 27 gauge air injection cannula (Bausch & Lomb Storz Ophthalmic, USA),attached to a 5mL syringe filled with air was introduced into the corneal tunnel. Air was then injected into the stroma to achieve the formation of a big-bubble (Figure 1C). The central anterior stromal disc was removed with an angled crescent knife, and then air was evacuated through the previously placed paracentesis.
To enter into the big-bubble, we used the Ophthalmic Viscosurgical Device–Assisted Incision technique⁵.A cohesive viscoelastic (Healon, Abbott Medical Optics) was placed on the stromal bed and a 1.0mm incision was then created with a paracentesis blade using only the tip of the blade with a “lifting” motion to prevent rapid collapse of the bubble which could result in Descemet membrane perforation. Entry into the big-bubble was confirmed by the appearance of a small bubble within the overlying viscoelastic material, which also served to prevent rapid egress of air from the big-bubble and rapid collapse leading to a higher probability of perforating the Descemet membrane (Figure 1D).
Entry into the big-bubble was confirmed by the appearance of a small bubble within the overlying viscoelastic material, which also served to prevent rapid egress of air from the big-bubble and rapid collapse leading to a higher probability of perforating the Descemet membrane (Figure 1D). Viscoelastic material was injected into the pre-Descemet space to expand the potential space and separate the Descemet membrane from the overlying stromal tissue.
The Bonfadini spatula (Katena Products, USA; Figure 2A) was introduced into the pre-Descemet space. This instrument has blunt edges and a curved profile to minimize inadvertent damage to Descemet membrane. The groove along the length of the Bonfadini spatula serves as a guide to allow for rapid incision of the posterior stromal tissue using the sharp point of a paracentesis blade (Figure 2B). The spatula serves to protect the DM from inadvertent perforation while incising the posterior stromal tissue. Once sufficient radial incisions in the posterior stroma were completed, we removed stroma with cornealscleral scissors along the peripheral partial thickness groove incision. The full-thickness donor graft was punched at 8.75mm diameter from the endothelial side, and the endothelium was stripped from the posterior surface using surgical spears. The donor button was then sutured into position with 16 interrupted 10-0 nylon sutures (Figure 2C).
The patient achieved uncorrected visual acuity of 20/125 and 20/60 with pinhole on day 1 postoperatively with a well-attached graft. At 3 months after DALK, his uncorrected visual acuity was 20/60 and best spectacle corrected visual acuity (-1.25 + 1.5 x 30 degrees) was 20/25 OD.
 
DISCUSSION
Corneal transplantation is singular because it is habitually performed on persons with visual deficiency but with preserved life expectancy, mobility and social life. A graft not well succeeded may cause real blindness and permanent misery due to pain and discomfort.⁶To obtain a satisfactory surgical result, there is a need of an appropriate patient selection and guidance about their eye problem, the proposed surgery, care and risk per and post operative as well as the visual rehabilitation perspective.⁷
Keratoconus is one of the main indications of keratoplasty in Brazil⁸, and DALK is more cost-effective than penetrating keratoplasty (PK).⁹Prevention of immune-mediated graft rejection can be achieved through meticulous surgical technique such as lamellar surgery.¹⁰
DALK aims to remove and replace total or near-total corneal stroma while preserving host healthy endothelium. The advantages of DALK include reducing the risk of endothelial graft rejection, preservation of host endothelium with minimal surgical trauma, efficient visual rehabilitation relative to penetrating keratoplasty (PK), and also fewer intraoperative and postoperative complications including expulsive hemorrhage, anterior synechia, postoperative endophthalmitis, and glaucoma in comparison to PK. This procedure also requires less rigid criteria for donor corneal tissue selection that is often weighted toward donor endothelium in PK.¹¹
Major disadvantages of anterior lamellar keratoplasty as compared to penetrating keratoplasty are the irregularity and sub-optimal optics of the corneal stromal bed which occur following manual lamellar dissection techniques.¹² These issues are avoided in DALK. However, the challenge with DALK continues to be the learning curve for novice surgeons when trying to expose DM (descemetic DALK [dDALK]) versus dissection in a pre-Descemetic stromal plane (pre-Descemetic DALK [pdDALK]).³ The dDALK procedure allows faster visual recovery than pdDALK.¹³ Thus, the goal of DALK is to expose the DM without damage and to achieve dDALK.
Lamellar dissection of the stroma can be performed by a manual technique using a variety of instruments, including lamellar knives and dissectors.¹⁴ Using the Bonfadini dissector, we could create a deep tunnel near the corneal center prior to air injection. The Bonfadini dissector has a fine, rounded tip, which can dissect stromal tissue relatively easily while reducing the likelihood of penetrating Descemet membrane. Accessing the deep stroma for air injection may improve the success of achieving the big-bubble.
The most serious complication during big-bubble DALK surgery is intraoperative perforation of Descemet membrane, which may require subsequent conversion to full-thickness penetrating keratoplasty.¹⁵Perforation of Descemet membrane is more likely to occur as a result of direct needle trauma during initial air injection and dissection of the corneal stroma.¹⁶ As well, perforation of DM can occur while opening the big-bubble and dissecting remaining stroma over Descemet membrane.
In the technique described here, viscoelastic material is placed on the stromal bed before opening the big-bubble.⁵ We can detect entry into the big-bubble (BB) by air leaking into the viscoelastic material. This sign allows a very controlled entry into the BB as it is readily and immediately visible and prevents rapid egress of air and collapse of the BB. Once the BB is accessed and further expanded by injection of viscoelastic into the BB, the design of the Bonfadini spatula allows it to be manipulated within the BB space with minimal chance of inadvertent trauma to DM.
The groove along the length of the Bonfadini spatula also serves as a convenient guide to facilitate rapid incision of the posterior stroma into wedges, which can be excised at the periphery using standard corneal scissors. Thus, we present the novel Bonfadini dissector and spatula as aids for the successful completion of DALK surgery. In summary, the benefits of our proposed technique could be shown more conclusively in a case–control or prospective study with a larger number of patients to validate our findings.
 
REFERENCES

1. Shimmura S, Tsubota K. Deep anterior lamellar keratoplasty. Curr Opin Ophthalmol. 2006;17(4):349-55. Review.

2. Michieletto P, Balestrazzi A, Balestrazzi A, Mazzotta C, Occhipinti I, Rossi T. Factors predicting unsuccessful big bubble deep lamellar anterior keratoplasty. Ophthalmologica. 2006;220(6):379-82.
3. Sarnicola V, Toro P. Blunt cannula for descemetic deep anterior lamellar keratoplasty. Cornea. 2011;30(8):895-8.
4. Anwar M, Teichmann KD. Big-bubble technique to bare Descemet’s membrane in
anterior lamellar keratoplasty. J Cataract Refract Surg. 2002;28(3):398-403.
5. Goshe J, Terry MA, Shamie N, Li J. Ophthalmic viscosurgical device-assisted incision modification for the big-bubble technique in deep anterior lamellar keratoplasty. J Cataract Refract Surg. 2011;37(11):1923-7.
6. Marcomini LA, Sobral RM, Seixas GO, Sousa SJ. [Corneal selection for transplants]. Rev Bras Oftalmol. 2011;70(6):430-6. Portuguese.
7. Kara-Junior N, Mourad PC, Espíndola RF, AbilRuss HH. [Expectation and knowledge among patients with keratoplasty indication]. Rev Bras Oftalmol. 2011;70(4):230-4. Portuguese.
8. Zeschau A, Balestrin IG, Stock RA, Bonamigo EL. [Indications of keratoplasty: a retrospective study in a University Hospital]. Rev Bras Oftalmol. 2013;72(5):316-20. Portuguese.
9. Koo TS, Finkelstein E, Tan D, Mehta JS. Incremental cost-utility analysis of deep anterior lamellar keratoplasty compared with penetrating keratoplasty for the treatment of keratoconus. Am J Ophthalmol. 2011;152(1):40-47.e2.
10. Costa DC, Kara-José N. [Corneal transplant rejection]. Rev Bras Oftalmol. 2008; 67(5):255-63. Portuguese.
11. Espandar L, Carlson AN. Lamellar Keratoplasty: A Literature Review. J Ophthalmol. 2013;2013:894319. Review.
12. Parthasarathy A, Por YM, Tan DT. Use of a “small-bubble technique” to increase the success of Anwar’s “big-bubble technique” for deep lamellar keratoplasty with complete baring of Descemet’s membrane. Br J Ophthalmol. 2007;91(10):1369-73.
13. Sarnicola V, Toro P, Gentile D, Hannush SB. Descemetic DALK and predescemetic DALK: outcomes in 236 cases of keratoconus. Cornea. 2010;29(1):53-9.
14. Bonfadini G, Moreira H, Jun AS, Campos M, Kim EC, Arana E, et al. Modified femtosecond laser-assisted sutureless anterior lamellar keratoplasty. Cornea. 2013;32(4):533-7.
15. Jhanji V, Sharma N, Vajpayee RB. Intraoperative perforation of Descemet’s membrane during “big bubble” deep anterior lamellar keratoplasty. Int Ophthalmol.
2010;30(3):291-5.
16. Leccisotti A. Descemet’s membrane perforation during deep anterior lamellar keratoplasty: prognosis. J Cataract Refract Surg. 2007;33(5):825-9.
 

Figure Legends

 
Figure 1. The Bonfadini dissector has a fine, rounded tip to enable stromal dissection while preventing inadvertent perforation of Descemet membrane (A). Use of the Bonfadini dissector to create a deep tunnel toward the center of the cornea from a peripheral partial thickness groove incision (B). Big-bubble formation by deep, intrastromal air injection with accompanying stromal opacification (C). Opening of the big-bubble with a sharp blade was detected by air leaking into viscoelastic material placed on the posterior stromal bed (D).

 
  
Figure 2. The groove along the Bonfadini spatula serves as a guide to protect Descemet membrane (DM) from inadvertent perforation when incising the posterior stroma with a blade (A).  Creation of wedge-shaped incisions of posterior stroma, using a blade guided along the groove of the Bonfadini spatula. The spatula serves to protect inadvertent perforation of DM (B). Donor button was sutured into position with 16 interrupted 10-0 nylon sutures (C).